A Reciprocal Regulation Of Spermidine And Autophagy In
Dec 1 2020 nbsp 0183 32 Podocytes display high levels of autophagy in vivo as indicated by LC3 fluorescence in mice transgenic for GFP LC3 a key marker for autophagosomes Figure 1a
A Reciprocal Regulation Of Spermidine And Autophagy In Podocytes , We describe metabolic signaling via spermidine as a key regulatory axis for autophagy activation in podocytes Our data highlight that auto phagy alteration as seen in focal segmental
Structure based Engineering Of Anti GFP Nanobody Tandems As
Apr 10 2020 nbsp 0183 32 We designed 3 linkers of different lengths to connect the GFP enhancer and LaG16 nanobody and tested their binding affinity to GFP
Structural Insights Into Two Distinct Nanobodies Recognizing The , Aug 6 2021 nbsp 0183 32 Our results provide atomic scale binding information for Nb2 sfGFP which is important for the further development of GFP nanobody based fusion protein manipulation
Cryo EM Structure Determination Of Small Proteins By Nanobody
Cryo EM Structure Determination Of Small Proteins By Nanobody , Here we describe a versatile method that allows cryo EM analysis of even the smallest protein once a tightly binding nanobody is available The size of the nanobody is increased to 120
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Influence Of Nanobody Binding On Fluorescence Emission
Influence Of Nanobody Binding On Fluorescence Emission Here we tested commercially available nanobodies and observed clear changes of the fluorescence properties mobility and organization of green fluorescent protein GFP tagged
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We now report a novel activity of transiently transfected SSAT1 in suppressing the exogenous expression of other proteins i e green fluorescent protein GFP or GFP eIF5A Suppression Of Exogenous Gene Expression By Spermidine . Here we describe a versatile method that allows cryo EM analysis of even the smallest protein once a tightly binding nanobody is available The size of the nanobody is increased to 120 kDa Here we report the cryo electron microscopy structures of human VPAT in complex with spermine spermidine H or tetrabenazine elucidating discrete lumen facing states of the
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